Asymmetric split-ring plasmonic nanostructures for the optical sensing of Escherichia coli.

Strategies for in-liquid micro-organism detection are crucial for the clinical and pharmaceutical industries. While Raman spectroscopy is a promising label-free technique for micro-organism detection, it remains challenging due to the weak bacterial Raman signals. In this work, we exploit the unique electromagnetic properties of metamaterials to identify bacterial components in liquid using an array of Fano-resonant metamolecules. This Fano-enhanced Raman scattering (FERS) platform is designed to exhibit a Fano resonance close to the protein amide group fingerprint around 6030 nm. Raman signatures of Escherichia coli were recorded at several locations on the metamaterial under off-resonance laser excitation at 530 nm, where the photodamage effect is minimized. As the sizes of the Escherichia coli are comparable to the micro-gaps i.e, 0.41 µm, of the metamaterials, its local immobilisation leads to an increase in the Raman sensitivity. We also observed that the time-dependent FERS signal related to bacterial amide peaks increased during the bacteria's mid-exponential phase while it decreased during the stationary phase. This work provides a new set of opportunities for developing ultrasensitive FERS platforms suitable for large-scale applications and could be particularly useful for diagnostics and environmental studies at off-resonance excitation.

A comprehensive evaluation of private sector investment decisions for sustainable water supply systems using a fuzzy-analytic hierarchy process: A case study of Ha Nam province in Vietnam.

This study examined the factors that affect the private sectors' willingness to invest in rural water supply. The study applied a mixed methods approach, including an overview of relevant studies, expert consultation, exploratory factor analysis using SPSS software, and a fuzzy-analytic hierarchy process to identify and evaluate the factors applicable to Ha Nam province in Vietnam. Some factors were distinguished that are significant to private investors' rural water supply investment decisions, including tax incentive policy, policies to support preferred access to loans and credit, a state risk-sharing mechanism, a mechanism to adjust water price, community support, high community demand for clean water, and input water quality. In addition, the study constructed an investment attractiveness index to evaluate the attractiveness of private sector investment for two typical rural water supply projects in Ha Nam province. This index can be used as a basis for the government to design appropriate incentives to attract investment from private investors and construct an investment attractiveness map.

Rapidly Determine the Maximum Power Point in the Parallel Configuration of the Photovoltaic System.

The maximum power point tracking (MPPT) solutions improve power generation efficiency, quickly stabilizing the output waveform of photovoltaic (PV) systems under variable operating conditions. Along with new algorithms, improved and adjusted methods to exploit energy from PV systems are increasingly being researched and proposed. However, the proposed solutions based on the traditional algorithms and their improvements have poor performance, while the advanced algorithms or hybrid methods bring high performance but need to be simplified, and the response speed is higher. Moreover, a suitable PV configuration makes choosing a simple but highly efficient algorithm, especially in low-power PV system applications such as rooftop solar power, traffic lights, and moving vehicles…where the number of PV panels is insufficient to implement flexible configurations. This paper proposes a modified version of the Perturb and Observe (MPO) algorithm to improve MPPT performance and increase convergence speed in the parallel structure of PV panels. The Short-Circuit Current (Isc) and Open-Circuit Voltage (Voc) are calculated directly at specific operating conditions to quickly determine the potential maximum power point (MPP) that will reduce power interruptions and increase power generation efficiency compared to periodic updates. Therefore, the proposed solution converges faster, with higher efficiency, and the output signal in static and dynamic MPPT situations is more stable. The results show that the highest efficiency in simulation and experiment is 99.99% and 99.93%, respectively, while the convergence speed is 0.01 s and 0.03 s, respectively. They are better than the traditional Perturb and Observe (P&O) algorithm, the Variable Step Size Perturb and Observe (VSSP&O) method, and the Particle Swarm Optimization (PSO) technique under the same operating conditions. In addition, its performance and convergence speed are also compared with the latest introduced algorithms. The results show that it is valuable and reliable for parallel PV configuration.

Predictive Factors of Mortality in Patients with Severe COVID-19 Treated in the Intensive Care Unit: A Single-Center Study in Vietnam.

INTRODUCTION: The fourth outbreak of COVID-19 with the delta variant in Vietnam was very fierce due to the limited availability of vaccines and the lack of healthcare resources. During that period, the high mortality of patients with severe and critical COVID-19 caused many concerns for the health system, especially the intensive care units. This study aimed to analyze the predictive factors of death and survival in patients with severe and critical COVID-19. METHODS: We conducted a cross-sectional and descriptive study on 151 patients with severe and critical COVID-19 hospitalized in the Intensive Care Unit of Binh Duong General Hospital. RESULTS: Common clinical symptoms of severe and critical COVID-19 included shortness of breath (97.4%), fatigue (89.4%), cough (76.8%), chest pain (47.7%), loss of smell (48.3%), loss of taste (39.1%), and headache (21.2%). The abnormal biochemical features were leukopenia (2.1%), anemia, thrombocytopenia (18%), hypoxia with low PaO2 (34.6%), hypocapnia with reduced PaCO2 (29.6%), and blood acidosis (18.4%). Common complications during hospitalization were septic shock (15.2%), cardiogenic shock (5.3%), and embolism (2.6%). The predictive factors of death were being female, age > 65 years, cardiovascular comorbidity, thrombocytopenia (< 137.109/l), and hypoxia at inclusion or after the first week or blood acidosis (pH < 7.28). The use of a high dose of corticosteroids reduced the mortality during the first 3 weeks of hospitalization but significantly increased risk of death after 3 and 4 weeks. CONCLUSIONS: Common clinical symptoms, laboratory features, and death-related complications of critical and severe COVID-19 patients were found in Vietnamese patients during the fourth wave of the COVID-19 pandemic. The results of this study provide new insight into the predictive factors of mortality for patients with severe and critical COVID-19.

Chromogenic enzyme substrates based on [2-(nitroaryl)ethenyl]pyridinium and quinolinium derivatives for the detection of nitroreductase activity in clinically important microorganisms.

A series of [2-(nitroaryl)ethenyl]pyridinium and quinolinium derivatives have been synthesised as potential indicators of microbial nitroreductase activity. When assessed against a selection of 20 clinically important pathogenic microorganisms, microbial colonies of various colours (yellow, green, red, brown, black) were produced and attributed to nitroreductase activity. Most substrates elicited colour responses with Gram-negative microorganisms. In contrast, the growth of several species of Gram-positive microorganisms and yeasts was often inhibited by the substrates and hence coloured responses were not seen.

Evanescent field trapping and propulsion of Janus particles along optical nanofibers.

Small composite objects, known as Janus particles, drive sustained scientific interest primarily targeted at biomedical applications, where such objects act as micro- or nanoscale actuators, carriers, or imaging agents. A major practical challenge is to develop effective methods for the manipulation of Janus particles. The available long-range methods mostly rely on chemical reactions or thermal gradients, therefore having limited precision and strong dependency on the content and properties of the carrier fluid. To tackle these limitations, we propose the manipulation of Janus particles (here, silica microspheres half-coated with gold) by optical forces in the evanescent field of an optical nanofiber. We find that Janus particles exhibit strong transverse localization on the nanofiber and much faster propulsion compared to all-dielectric particles of the same size. These results establish the effectiveness of near-field geometries for optical manipulation of composite particles, where new waveguide-based or plasmonic solutions could be envisaged.

Particle trapping with optical nanofibers: a review [Invited].

Optical trapping has proven to be an efficient method to control particles, including biological cells, single biological macromolecules, colloidal microparticles, and nanoparticles. Multiple types of particles have been successfully trapped, leading to various applications of optical tweezers ranging from biomedical through physics to material sciences. However, precise manipulation of particles with complex composition or of sizes down to nanometer-scales can be difficult with conventional optical tweezers, and an alternative manipulation tool is desirable. Optical nanofibers, that is, fibers with a waist diameter smaller than the propagating wavelength of light, are ideal candidates for optical manipulation due to their large evanescent field that extends beyond the fiber surface. They have the added advantages of being easily connected to a fibered experimental setup, being simple to fabricate, and providing strong electric field confinement and intense magnitude of evanescent fields at the nanofiber's surface. Many different particles have been trapped, rotated, transported, and assembled with such a system. This article reviews particle trapping using optical nanofibers and highlights some challenges and future potentials of this developing topic.

Exploring potential machine learning application based on big data for prediction of wastewater quality from different full-scale wastewater treatment plants.

Water pollution generated from intensive anthropogenic activities has emerged as a critical issue concerning ecosystem balance and livelihoods worldwide. Although optimizing wastewater treatment efficiency is widely regarded as the foremost step to minimize pollutants released into the environment, this widespread application has encountered two major problems: firstly, the significant variation of influent wastewater constituents; secondly, complex treatment processes within wastewater treatment plants (WWTPs). Based on the data collected hourly using real-time sensors in three different full-scale WWTPs (24 h × 365 days × 3 WWTPs × 10 wastewater parameters), this work introduced the potential application of Machine Learning (ML) to predict wastewater quality. In this work, six different ML algorithms were examined and compared, varying from shallow to deep learning architectures including Seasonal Autoregressive Integrated Moving Average (SARIMAX), Random Forest (RF), Support Vector Machine (SVM), Gradient Tree Boosting (GTB), Adaptive Neuro-Fuzzy Inference System (ANFIS) and Long Short-Term Memory (LSTM). These models were developed to detect total phosphorus in the outlet (Outlet-TP), which served as an output variable due to the rising concerns about the eutrophication problem. Irrespective of WWTPs, SARIMAX consistently demonstrated the best performance for regression estimation as evidenced by the lowest values of Mean Square Error (MSE), Mean Absolute Error (MAE), Mean Absolute Percentage Error (MAPE) and the highest coefficient of determination (R2). In terms of computation efficiency, SARIMAX exhibited acceptable time computation, acknowledging the successful application of this algorithm for Outlet-TP modeling. In contrast, the complex structure of LSTM made it time-consuming and unstable coupled with noise, while other shallower architectures, i.e., RF, SVM, GTB, and ANFIS were unable to address large datasets with nonlinear and nonstationary behavior. Consequently, this study provides a reliable and accurate approach to forecast wastewater effluent quality, which is pivotal in terms of the socio-economic aspects of wastewater management.

Impact of Infectious Disease after Lactococcus lactis Strain Plasma Intake in Vietnamese Schoolchildren: A Randomized, Placebo-Controlled, Double-Blind Study.

Lactococcus lactis strain Plasma (LC-Plasma) is reported to have anti-viral effects via direct activation of plasmacytoid dendritic cells, which upregulate the production of type I and III interferons. A randomized, placebo-controlled, double-blind, parallel group study was designed for elementary schoolchildren, grades 1 to 3, in Vietnam. LC-Plasma or a control were administered to schoolchildren as a beverage (1.0 × 1011 count LC-Plasma/day/person). The primary endpoint was to determine the efficacy of LC-Plasma in reducing the cumulative days absent from school due to upper respiratory disease (URID) and gastrointestinal disease (GID), and the secondary endpoint was to evaluate the potency of LC-Plasma on URID/GID symptoms and general well-being scores. LC-Plasma intake significantly reduced the cumulative days absent from school due to URID/GID (Odds ratio (OR) = 0.57, p = 0.004) and URID alone (OR = 0.56, p = 0.005); LC-Plasma also significantly reduced the number of cumulative fever positive days during the first 4 weeks of intervention (OR = 0.58, p = 0.001) and cumulative days with diarrhea during the last 4 weeks of the intervention period (OR = 0.78, p = 0.01). The number of positive general wellbeing days was significantly improved in the LC-Plasma group compared with the control throughout the intervention period (OR = 0.93, 0.93, p = 0.03, 0.04 in the first and last 4 weeks of the intervention, respectively). These data suggest that LC-Plasma seems to improve the health condition of elementary schoolchildren and reduces school absenteeism due to infectious disease, especially URID.

Sero-Prevalence of SARS-CoV-2 Antibodies in High-Risk Populations in Vietnam.

As a response to the coronavirus disease 2019 (COVID-19) pandemic, Vietnam enforced strict quarantine, contact tracing and physical distancing policies resulting in one of the lowest numbers of individuals infected with severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) globally. This study aimed to determine the prevalence of SARS-CoV-2 antibody positivity among high-risk populations in Vietnam. A prevalence survey was undertaken within four communities in Vietnam, where at least two COVID-19 cases had been confirmed. Participants were classified according to the location of exposure: household contacts, close contacts, community members, and healthcare workers (HCWs) responsible for treating COVID-19 cases. Participants completed a baseline questionnaire and SARS-CoV-2 IgG antibodies were quantified using a commercial assay. A total of 3049 community members and 149 health care workers consented to the study. Among 13 individuals who were seropositive (0.4%), five household contacts (5/27, 18.5%), one close contact (1/53, 1.9%), and seven community members (7/2954, 0.2%) had detectable SARS-CoV-2 antibodies. All HCWs were negative for SARS-CoV-2 antibodies. Participants were tested a median of 15.1 (interquartile range from 14.9 to 15.2) weeks after exposure. Our study found a low prevalence of SARS-CoV-2 antibodies in high-risk communities and healthcare workers in communities in Vietnam with known COVID-19 cases.

Preconception micronutrient supplementation positively affects child intellectual functioning at 6 y of age: A randomized controlled trial in Vietnam.

BACKGROUND: Although there is growing evidence on the role of preconception nutrition for birth outcomes, very few studies have evaluated the long-term effects of nutrition interventions during the preconception period on offspring cognitive outcomes. OBJECTIVE: We evaluate the impact of preconception weekly multiple micronutrients (MMs) or iron and folic acid (IFA) supplementation compared with folic acid (FA) alone on offspring intellectual functioning at age 6-7 y. METHODS: We followed 1599 offspring born to women who participated in a double-blinded randomized controlled trial of preconception supplementation in Vietnam. Women received weekly supplements containing either 2800 µg FA only, 60 mg iron and 2800 µg FA, or MMs (15 micronutrients including IFA) from baseline until conception, followed by daily prenatal IFA supplements until delivery. We used the Wechsler Intelligence Scale for Children to measure full-scale IQ (FSIQ) and 4 related domains of intellectual functioning [Verbal Comprehension Index (VCI), Perceptual Reasoning Index (PRI), Working Memory Index (WMI), and Processing Speed Index (PSI) scores] at 6-7 y. Group comparisons were done using ANOVA tests for all children and the subgroup born to women who consumed the supplements ≥26 wk before conception (per-protocol analyses). RESULTS: The final sample with data at 6-7 y (n = 1321) was similar for baseline maternal and offspring birth characteristics and age at follow-up by treatment group. Compared with the offspring in the FA group, those in the MM group had higher FSIQ (ß = 1.7; 95% CI: 0.1, 3.3), WMI (ß = 1.7; 95% CI: 0.2, 3.2), and PSI (ß = 2.5; 95% CI: 0.9, 4.1). Similar findings were observed in the per-protocol analyses. There were no significant differences by treatment group for VCI and PRI. CONCLUSIONS: Preconception supplementation with MMs improved certain domains of intellectual functioning at age 6-7 y compared with FA. These findings suggest the potential for preconception micronutrient interventions to have long-term benefits for offspring cognition.

Efficacy of probiotics on digestive disorders and acute respiratory infections: a controlled clinical trial in young Vietnamese children.

OBJECTIVES: To evaluate the efficacy of fermented milk containing Lactobacillus casei strain Shirota (LcS) on the incidence of constipation, diarrhea, acute respiratory infections (ARI), and nutritional status of young Vietnamese children. METHODS: A controlled field trial was conducted with 1003 children (3-5 years old) in Thanh Hoa province in Vietnam. The probiotic group (n = 510) consumed fermented milk 65 mL/day containing 108 CFU/mL of LcS for the 12-week intervention period, whereas the control group (n = 493) was not given any. The incidence of constipation, diarrhea, ARI, and anthropometry in children was determined at baseline, after 4, 8, and 12-week intervention, and after the 4-week follow-up period. RESULTS: Probiotic drink decreased the incidence of constipation after the 12-week intervention period (12.0% vs. 32.0%, OR = 0.28 (95% CI: 0.21-0.40), p < 0.001), tended to decrease the incidence of diarrhea (4.9% vs. 7.9%, OR = 0.60 (95% CI: 0.35-1.01), p = 0.068), and prevented the occurrence of ARI (15.9% vs. 24.5%, OR = 0.58 (95% CI: 0.42-0.79), p < 0.001), when compared with the control group. In contrast, no probiotic effects were observed for the duration of diarrhea or ARI. Weight gain was higher in the probiotic group than in the control group after 4, 8, and 12-week intervention and after the 4-week follow-up period (p < 0.05). CONCLUSIONS: Daily intake of fermented milk containing LcS strongly prevented the incidence of constipation and ARI in Vietnamese children. This study also revealed the potential effects of the use of a probiotic drink on diarrhea prevention as well as nutritional status improvement.

Fast and efficient nanoparticle trapping using plasmonic connected nanoring apertures.

The manipulation of microparticles using optical forces has led to many applications in the life and physical sciences. To extend optical trapping towards the nano-regime, in this work we demonstrate trapping of single nanoparticles in arrays of plasmonic coaxial nano-apertures with various inner disk sizes and theoretically estimate the associated forces. A high normalized experimental trap stiffness of 3.50 fN nm-1 mW-1 µm-2 for 20 nm polystyrene particles is observed for an optimum design of 149 nm for the nanodisk diameter at a trapping wavelength of 980 nm. Theoretical simulations are used to interpret the enhancement of the observed trap stiffness. A quick particle trapping time of less than 8  s is obtained at a concentration of 14 × 1011 particles ml-1 with low incident laser intensity of 0.59 mW µm-2. This good trapping performance with fast delivery of nanoparticles to multiple trapping sites emerges from a combination of the enhanced electromagnetic near-field and spatial temperature increase. This work has applications in nanoparticle delivery and trapping with high accuracy, and bridges the gap between optical manipulation and nanofluidics.

The beneficial role of FeNO in association with GINA guidelines for titration of inhaled corticosteroids in adult asthma: A randomized study.

PURPOSE: This study aimed to demonstrate the role of fractional concentration of exhaled nitric oxide (FeNO) in association with Global Initiative for Asthma (GINA) guidelines for treatment of adult patients with asthma. METHODS: It was a prospective and randomized study. The symptomatic asthmatic patients were randomly divided into two groups: GINA group (followed GINA guidelines; N = 86) or GINA + FeNO group (followed GINA guidelines + FeNO for titration of inhaled corticosteroids - ICS; N = 90). They were followed-up for 9 months. RESULTS: In GINA group, 37.2% patients had no treatment and 62.8% patients discontinued treatment vs. 40.0% and 60.0% in GINA + FeNO, respectively. After 3, 6 and 9 months of treatment, the percentage of mild, moderate and severe asthma showed no significant difference between the two groups. At 9th month, Δ moderate asthma (reduction) in GINA + FeNO group was significantly higher than in the GINA group (-22.0% vs. -11.6%; P = 0.018). The improvement of asthma control test (ACT) score was not different between the groups at 9th month (12 ± 6 vs. 10 ± 5; P > 0.05); the level of FeNO reduction in GINA + FeNO group was significantly higher than that in GINA group (-42 ± 11 vs. -35 ± 9; P = 0.022). The daily dose of ICS in GINA + FeNO group was significantly lower than that in GINA group (397 ± 171 vs. 482 ± 240 mcg and 375 ± 203 vs. 424 ± 221 mcg; respectively) at the end of 6 and 9 months. CONCLUSION: The use of FeNO in association with GINA guidelines has a beneficial role for accurate daily dose of ICS in adult patients with asthma.

Fano-Resonant, Asymmetric, Metamaterial-Assisted Tweezers for Single Nanoparticle Trapping.

Plasmonic nanostructures overcome Abbe's diffraction limit to create strong gradient electric fields, enabling efficient optical trapping of nanoparticles. However, it remains challenging to achieve stable trapping with low incident laser intensity. Here, we demonstrate Fano resonance-assisted plasmonic optical tweezers for single nanoparticle trapping in an array of asymmetrical split nanoapertures on a 50 nm gold thin film. A large normalized trap stiffness of 8.65 fN/nm/mW for 20 nm polystyrene particles at a near-resonance trapping wavelength of 930 nm was achieved. The trap stiffness on-resonance is enhanced by a factor of 63 compared to that of off-resonance due to the ultrasmall mode volume, enabling large near-field strengths and a cavity effect contribution. These results facilitate trapping with low incident laser intensity, thereby providing new options for studying transition paths of single molecules such as proteins.

Breast Self-Examination: Knowledge and Practice Among Female Textile Workers in Vietnam.

Breast self-examination (BSE) is a simple, feasible, and suitable method for breast cancer screening in low-middle setting countries. The aim of this study was to assess the knowledge and practice of BSE among female textile workers in Vietnam. A cross-sectional study was conducted among 1036 female workers, aged 43.9 ± 3.1 years old, who were working in 4 textile enterprises in Hanoi and Ho Chi Minh City, in 2016. Overall, 22.7% of participants showed sufficient knowledge on BSE; 15.2% performed monthly BSE; and 7.7% completely performed all 5 steps of BSE. The prevalence of insufficient BSE knowledge was higher among participants with low level of education (odds ratio [OR]: 1.71; 95% confidence interval [CI]: 1.22-2.39, below vs above secondary school), and those whose husbands were farmers or workers (OR: 1.76; 95% CI: 1.27-2.45, compared to other sectors). This prevalence was, however, negatively associated with receiving information on BSE (OR: 0.16; 95% CI: 0.11-0.23, yes vs no). The prevalence of insufficient BSE practice was also higher among females with husbands who were farmers or workers (OR: 1.67; 95% CI: 1.15-2.43, compared to other sectors). In contrast, it was much lower among participants who had history of breast-related diseases (OR: 0.46; 95% CI: 0.24-0.88, yes vs no), received BSE information (OR: 0.13; 95% CI: 0.08-0.19, yes vs no), and had sufficient overall BSE knowledge (OR: 0.09; 95% CI: 0.06-0.13, yes vs no), compared to those who did not. There was a low prevalence of sufficient BSE knowledge (22.7%) and practice (15.8%) among female textile workers in Hanoi and Ho Chi Minh City. Low level of education and not being provided BSE information were associated with insufficient BSE knowledge and practice among Vietnamese female textile workers. Health education programs are essential to encourage and improve women's knowledge and practice of BSE.

Demand and willingness to pay for different treatment and care services among patients with heart diseases in Hanoi, Vietnam.

INTRODUCTION: In Vietnam, cardiovascular diseases (CVDs) are serious health issues, especially in the context of overload central heart hospitals, insufficient primary healthcare, and lack of customer-oriented care and treatment. Attempts to measure demand and willingness-to-pay (WTP) for different CVD treatments and care services have been limited. This study explored the preferences and WTP of patients with heart diseases for different home- and hospital-based services in Hanoi, Vietnam. METHODS: A cross-sectional survey was performed at the Hanoi Heart Hospital from July to December 2017. A contingent valuation was adopted to determine the preferences of patients and measure their WTP. Interval regressions were employed to determine the potential predictors of patients' WTP. RESULTS: Hospital-based services were most preferred by patients, with demand ranging from 45.6% to 82.3% of total participants, followed by home-based (45.4%-45.8%) and administrative services (28.9%-34%). WTP for hospital-based services were in the range of US$ 9.8 (US$ 8.4-11.2)-US$ 21.9 (US$ 20.3-23.4), while figures for home-based and administrative services were US$ 9.8 (US$ 8.4-11.2)-US$ 22 (US$ 18.7-25.3) and 1.9 (US$ 1.6-2.2)-US$ 7.5 (US$ 6.3-8.6), respectively. Patients who lived in urban areas, were employed, were having higher level of education, and were not covered by health insurance were willing to pay more for services, especially home-based ones. CONCLUSION: Demand and WTP for home-based services among heart disease patients were moderately low compared with hospital-based ones. There is a need for more policies supporting home-based services, better communication of services' benefits to general public and patients, and introduction of services packages based on patients' preferences.

Preconception Micronutrient Supplementation with Iron and Folic Acid Compared with Folic Acid Alone Affects Linear Growth and Fine Motor Development at 2 Years of Age: A Randomized Controlled Trial in Vietnam.

Background: Maternal health and nutrition play a crucial role in early child growth and development. However, little is known about the benefits of preconception micronutrient interventions beyond the role of folic acid (FA) and neural tube defects.Objective: We evaluated the impact of weekly preconception multiple micronutrient (MM) or iron and folic acid (IFA) supplementation on child growth and development through the age of 2 y compared with FA alone.Methods: We followed 1599 offspring born to women who participated in a randomized controlled trial of preconception supplementation in Vietnam. Women received weekly supplements that contained either 2800 µg FA, 60 mg Fe and 2800 µg FA, or 15 MMs including IFA, from baseline until conception followed by daily prenatal IFA supplements until delivery. Child anthropometry was measured at birth and at 3, 6, 12, 18, and 24 mo. Child development was measured with the use of the Bayley Scales for Infant Development III at 24 mo.Results: The groups were similar for baseline maternal and offspring birth characteristics. At 24 mo of age, the offspring in the IFA group had significantly higher length-for-age z scores (LAZs) (0.14; 95% CI: 0.03, 0.26), reduced risk of being stunted (0.87; 95% CI: 0.76, 0.99), and smaller yearly decline in LAZs (0.10; 95% CI: 0.04, 0.15) than the offspring in the FA group. Similar trends were found for the offspring in the MM group compared with the FA group for LAZs (0.10; 95% CI: -0.02, 0.22) and the risk of being stunted (0.88; 95% CI: 0.77, 1.01). Offspring in the IFA group had improved motor development (P = 0.03), especially fine motor development (0.41; 95% CI: 0.05, 0.77), at the age of 24 mo, but there were no differences for measures of cognition or language.Conclusions: Preconception supplementation with IFA improved linear growth and fine motor development at 2 y of age compared with FA. Future studies should examine whether these effects persist and improve child health and schooling. The trial was registered at clinicaltrials.gov as NCT01665378.

Persistence and compliance with newly initiated antihypertensive drug treatment in patients with chronic kidney disease.

BACKGROUND: Patients with chronic kidney disease initiating an antihypertensive drug (AH) treatment must persist and comply with it to slow disease progression and benefit from the reduction of cardiovascular morbidity and mortality. OBJECTIVES: This study evaluates the persistence and compliance with AH treatment and identifies the associated factors among chronic kidney disease patients who initiated AH treatment. METHODS: A population-based cohort study using Quebec administrative data was conducted. Patients who still take any AH 1 year after initiation were considered persistent. Of these patients, those who had ≥80% of days covered with an AH in the year after initiation were considered compliant. Factors associated with persistence and compliance were identified using a modified Poisson regression. RESULTS: Of the 7,119 eligible patients, 78.8% were persistent, 87.7% of whom were compliant with their AH treatment. Compared with patients on diuretic monotherapy, those who initially used angiotensin-converting enzyme inhibitor monotherapy, angiotensin II receptor blocker monotherapy, calcium channel blocker monotherapy, ß-blocker monotherapy, or multidrug therapy were more likely to be persistent. In contrast, individuals who visited their physicians ≥17 times were less likely to be persistent than those who visited between 0 and 8 times. The patients who were more likely to be compliant had initially used an angiotensin-converting enzyme inhibitor, ß-blocker, calcium channel blocker, or multitherapy as opposed to a diuretic. CONCLUSION: A year after initiating AH treatment, nearly a third of chronic kidney disease patients were either not taking an AH or had not been compliant. Factors associated with persistence and compliance could help identify patients who need help in managing their AH treatment.

Nonlinear force dependence on optically bound micro-particle arrays in the evanescent fields of fundamental and higher order microfibre modes.

Particles trapped in the evanescent field of an ultrathin optical fibre interact over very long distances via multiple scattering of the fibre-guided fields. In ultrathin fibres that support higher order modes, these interactions are stronger and exhibit qualitatively new behaviour due to the coupling of different fibre modes, which have different propagation wave-vectors, by the particles. Here, we study one dimensional longitudinal optical binding interactions of chains of 3 µm polystyrene spheres under the influence of the evanescent fields of a two-mode microfibre. The observation of long-range interactions, self-ordering and speed variation of particle chains reveals strong optical binding effects between the particles that can be modelled well by a tritter scattering-matrix approach. The optical forces, optical binding interactions and the velocity of bounded particle chains are calculated using this method. Results show good agreement with finite element numerical simulations. Experimental data and theoretical analysis show that higher order modes in a microfibre offer a promising method to not only obtain stable, multiple particle trapping or faster particle propulsion speeds, but that they also allow for better control over each individual trapped object in particle ensembles near the microfibre surface.